Quantitative Proteomics

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Quantitative Proteomics

The Quantitative Proteomics Center supports the ongoing research programs of collaborating laboratories.

Contact us through our website to start a new collaboration. Recent innovations in quantitative proteomics have demonstrated a variant of a label-free LC-MS (shotgun) approach in which mass spectra are recorded at alternate low (precursor) and high (product) fragmentation voltages in a method called MSE. In this approach rapidly alternating parent and product ion spectra are generated in a protoco

l that seeks to record "all the ions all the time, and these data are analyzed with an ion accounting algorithm. Increases in reproducibility of chromatographic separations have enhanced the feasibility of this approach. Building on these conceptual and instrumentation advances a number of groups have demonstrated effective use of this method for microbial cells (2), human serum (3), and cancer cells (4). Waters Corporation (Milford, MA) has implemented the MSE algorithm in a software routine known as IdentityE. We have used this system to study proteomes of Drosophila, yeast, bacterial (E. coli, Neisseria, Nitrosomonas), mouse, rat and human proteomes.

Address

Lewis M. Brown, Director, Quantitative Proteomics Center At Columbia University, Department Of Biological Sciences, MC2417, 1212 Amsterdam Avenue
New York, NY
10027

Telephone

+16465580007

Website

http://proteomics.bio.columbia.edu/

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