11/08/2026
Kexin Wang et al. Supplementation of the freezing extender with glutathione peroxidase improves the post-thaw quality of ram semen by enhancing antioxidant capacity, regulating metabolic enzyme activity, and modulating proteome expression. Animal Bioscience 2026; 39(8): 250985. [The study led by Profs. Shi Lei and Youshe Ren at Shanxi Agricultural University, Taigu, China]
https://doi.org/10.5713/ab.250985
The authors of the current study investigated the effects of different concentrations of glutathione peroxidase (GSH-Px) on the quality of frozen-thawed ram semen. Supplementation with 1.6 U/L GSH-Px significantly improved s***m motility, viability, membrane and DNA integrity, acrosome integrity, and mitochondrial activity. GSH-Px also enhanced antioxidant capacity by increasing SOD, CAT, and T-AOC while reducing oxidative stress markers such as MDA and ROS. Proteomic analysis further identified changes in several proteins, suggesting that GSH-Px supplementation improves post-thaw s***m quality by reducing oxidative damage and modulating protein expression.
The protocol for the present study was approved by Institutional Animal Care and Use Committee of Shanxi Agricultural University (SXAU-EAW-2022S.MN.012013378).