23/04/2026
🔬 High-speed, label-free 3D imaging of live cells with just 11 images
A team led by Prof. Qian Chen and Prof. Chao Zuo at Nanjing University of Science and Technology, together with collaborators at University of Technology Sydney, reports a new non-interferometric optical diffraction tomography method in Advanced Photonics.
The approach, known as spatiotemporal multiplexed Fourier ptychographic diffraction tomography (STM-FPDT), reconstructs 3D refractive index distributions from only 11 intensity images, achieving ~5 Hz imaging speed with high spatial resolution while suppressing motion artifacts.
This technique enables label-free visualization of subcellular dynamics, including membrane remodeling, vesicle transport, actin filament behavior, mitochondrial fission, and intercellular interactions.
These results provide a powerful new tool for studying complex biological processes in real time with minimal perturbation.
🔗 Read more in : https://www.spiedigitallibrary.org/journals/advanced-photonics/volume-8/issue-02/026003/Spatiotemporal-multiplexed-Fourier-ptychographic-diffraction-tomography-for-high-speed-label/10.1117/1.AP.8.2.026003.full